The purpose of ATP analogues
ATP as an important molecule has been known to play a vital role in the production of energy in humans, and is also involved in vital metabolisms that occur in humans. Simply put, the ATP analogues are employed when it’s time to probe biological systems or processes, and they’re created from biological processes. Analogs are made to look like an ATP biologically, but they possess certain traits that can give useful effects. Creating an ATP analogue used for probing involves chemical methods, and we’ll explain some chemical reactions that will produce ATP analogues.
One popular way of getting an ATP analogue is by modifying the ATP phosphate chain to make it impossible to undergo hydrolyzation which will change its activity. The first purpose this serves is that it’ll to used to out-compete ATP when added to a system, making effects of hydrolysis reduced.
You can also use it to trap any molecule of bimolecule in an ATP-bound state for a biochemical analysis which might not be possible before, as ATP is quickly hydrolyzed. It’s hard to produce an ATP analogue that is perfect, meaning that it’s possible to get your molecule trapped in an ATP intermediate stage, instead of the one you intended to. To reduce the chance of that happening, it’s good to use multiple ATP analogues.
Experts have shown that ATP can be modified with a probe that reports on the environment of the molecule. Analogs work as infinity labels to identify sites that bind nucleotides in protein primary structures. It’s used to separate proteins that consume ATP from other proteins. There are different ATP analogs, and each has a different purpose that it serves, for instance, the ATP analogue ADP-BeFx for structural or biological analysis. Experts have spent years to identify all the functions that these ATP analogs serve.